An Inexpensive Maximum Power Point Tracking System for an Insulated Solar Electric Cooker using an Off-the-Shelf Buck Converter

A specialized control circuit using an off-the-shelf buck converter is built for an Insulated Solar Electric Cooker (ISEC). Cost and efficient power delivery are the focus. An ISEC is synonymous to a direct load heat resistor, allowing a specific maximum power point tracking (MPPT) algorithm and fewer components. Only a microcontroller, voltage sensor, and digital-to-analog converter are used with the buck converter to maximize the power delivered by a 100W solar panel for the 3.3Ω load

Characterization of the ZFX family of transcription factors that bind downstream of the start site of CpG island promoters

Our study focuses on a family of ubiquitously expressed human C₂H₂ zinc finger proteins comprised of ZFX, ZFY and ZNF711. Although their protein structure suggests that ZFX, ZFY and ZNF711 are transcriptional regulators, the mechanisms by which they influence transcription have not yet been elucidated. We used CRISPR-mediated deletion to create bi-allelic knockouts of ZFX and/or ZNF711 in female HEK293T cells (which naturally lack ZFY). We found that loss of either ZFX or ZNF711 reduced cell growth and that the double knockout cells have major defects in proliferation. RNA-seq analysis revealed that thousands of genes showed altered expression in the double knockout clones, suggesting that these TFs are critical regulators of the transcriptome. To gain insight into how these TFs regulate transcription, we created mutant ZFX proteins and analyzed them for DNA binding and transactivation capability. We found that zinc fingers 11–13 are necessary and sufficient for DNA binding and, in combination with the N terminal region, constitute a functional transactivator. Our functional analyses of the ZFX family provides important new insights into transcriptional regulation in human cells by members of the large, but under-studied family of C₂H₂ zinc finger proteins

Stratospheric dynamics and midlatitude jets under geoengineering with space mirrors and sulfate and titania aerosols

The impact on the dynamics of the stratosphere of three approaches to geoengineering by solar radiation management is investigated using idealized simulations of a global climate model. The approaches are geoengineering with sulfate aerosols, titania aerosols, and reduction in total solar irradiance (representing mirrors placed in space). If it were possible to use stratospheric aerosols to counterbalance the surface warming produced by a quadrupling of atmospheric carbon dioxide concentrations, tropical lower stratospheric radiative heating would drive a thermal wind response which would intensify the stratospheric polar vortices. In the Northern Hemisphere this intensification results in strong dynamical cooling of the polar stratosphere. Northern Hemisphere stratospheric sudden warming events become rare (one and two in 65 years for sulfate and titania, respectively). The intensification of the polar vortices results in a poleward shift of the tropospheric midlatitude jets in winter. The aerosol radiative heating enhances the tropical upwelling in the lower stratosphere, influencing the strength of the Brewer-Dobson circulation. In contrast, solar dimming does not produce heating of the tropical lower stratosphere, and so there is little intensification of the polar vortex and no enhanced tropical upwelling. The dynamical response to titania aerosol is qualitatively similar to the response to sulfate

dCas9-based epigenome editing suggests acquisition of histone methylation is not sufficient for target gene repression.

Distinct epigenomic profiles of histone marks have been associated with gene expression, but questions regarding the causal relationship remain. Here we investigated the activity of a broad collection of genomically targeted epigenetic regulators that could write epigenetic marks associated with a repressed chromatin state (G9A, SUV39H1, Krüppel-associated box (KRAB), DNMT3A as well as the first targetable versions of Ezh2 and Friend of GATA-1 (FOG1)). dCas9 fusions produced target gene repression over a range of 0- to 10-fold that varied by locus and cell type. dCpf1 fusions were unable to repress gene expression. The most persistent gene repression required the action of several effector domains; however, KRAB-dCas9 did not contribute to persistence in contrast to previous reports. A 'direct tethering' strategy attaching the Ezh2 methyltransferase enzyme to dCas9, as well as a 'recruitment' strategy attaching the N-terminal 45 residues of FOG1 to dCas9 to recruit the endogenous nucleosome remodeling and deacetylase complex, were both successful in targeted deposition of H3K27me3. Surprisingly, however, repression was not correlated with deposition of either H3K9me3 or H3K27me3. Our results suggest that so-called repressive histone modifications are not sufficient for gene repression. The easily programmable dCas9 toolkit allowed precise control of epigenetic information and dissection of the relationship between the epigenome and gene regulation

Nuclear spirals as feeding channels to the Supermassive Black Hole: the case of the galaxy NGC 6951

We report the discovery of gas streaming motions along nuclear spiral arms towards the LINER nucleus of the galaxy NGC 6951. The observations, obtained using the GMOS integral field spectrograph on the Gemini North telescope, yielded maps of the flux distributions and gas kinematics in the Halpha, [NII]6584 and [SII]6717,31 emission lines of the inner 7x5 arcsec^2 of the galaxy. This region includes a circumnuclear star-forming ring with radius 500pc, a nuclear spiral inside the ring and the LINER nucleus. The kinematics of the ionized gas is dominated by rotation, but subtraction of a kinematic model of a rotating exponential disk reveals deviations from circular rotation within the nuclear ring which can be attributed to (1) streaming motions along the nuclear spiral arms and (2) a bipolar outflow which seems to be associated to a nuclear jet. On the basis of the observed streaming velocities and geometry of the spiral arms we estimate a mass inflow rate of ionized gas of 3x10^(-4) Msun/yr, which is of the order of the accretion rate necessary to power the LINER nucleus of NGC 6951. Similar streaming motions towards the nucleus of another galaxy with LINER nucleus -- NGC 1097 -- have been reported by our group in a previous paper. Taken together, these results support a scenario in which nuclear spirals are channels through which matter is transferred from galactic scales to the nuclear region to feed the supermassive black hole.Comment: 25 pages, 6 eps figures, accepted for publication in Ap

Renewable Integration in Island Electricity Systems – A System Dynamics Assessment

Island electricity systems tend to rely heavily on the use of fossil fuels for the everyday supply of customer needs, so there are both significant economic and environmental benefits from the decarbonisation of these systems. One such key global environmental benefit is the anticipated reduction in CO2 emissions and its associated effects on climate change. In recognition, many islands are already pursuing ambitious goals for renewable energy sources integration. The resulting effects of policy on the long-term investment decisions however, need to be better understood. This paper presents a system dynamics simulation model which evaluates the adoption and diffusion of renewable generation sources within an existing island electricity system. In particular, renewable sources within the Azorean island of São Miguel are considered, with findings revealing that the requisite long-term investments are framed by the local experience of the renewable technologies and the pursuit of further renewable integration policy targets

Characterization of the ZFX family of transcription factors that bind downstream of the start site of CpG island promoters

Our study focuses on a family of ubiquitously expressed human C₂H₂ zinc finger proteins comprised of ZFX, ZFY and ZNF711. Although their protein structure suggests that ZFX, ZFY and ZNF711 are transcriptional regulators, the mechanisms by which they influence transcription have not yet been elucidated. We used CRISPR-mediated deletion to create bi-allelic knockouts of ZFX and/or ZNF711 in female HEK293T cells (which naturally lack ZFY). We found that loss of either ZFX or ZNF711 reduced cell growth and that the double knockout cells have major defects in proliferation. RNA-seq analysis revealed that thousands of genes showed altered expression in the double knockout clones, suggesting that these TFs are critical regulators of the transcriptome. To gain insight into how these TFs regulate transcription, we created mutant ZFX proteins and analyzed them for DNA binding and transactivation capability. We found that zinc fingers 11–13 are necessary and sufficient for DNA binding and, in combination with the N terminal region, constitute a functional transactivator. Our functional analyses of the ZFX family provides important new insights into transcriptional regulation in human cells by members of the large, but under-studied family of C₂H₂ zinc finger proteins